|
KMID : 0881720190340030277
|
|
Journal of Food Hygiene and Safety
2019 Volume.34 No. 3 p.277 ~ p.282
|
|
|
Comparison of Loop-mediated Isothermal Amplification and Korea Standard Food Codex (KFSC) Method for Detection of Salmonella Typhimurium, Listeria monocytogenes Artificially Inoculated in Yuk-hwe and Yuk-sashimi
|
|
Kwak Seung-Hae
Lee So-Young
Kim Jin-Hee
Oh Se-Wook
|
|
|
Abstract
|
|
|
|
The object of this study is to compare the performance of the 3M Molecular Detection Assay 2 (3M MDA 2) and the Korea Standard Food Codex (KSFC) Method (i.e., isolation media and real-time PCR) in detect-ing Salmonella Typhimurium and Listeria monocytogenes in traditional Korean foods. Yuk-hwe and Yuk-sashimi (types of raw beef dishes) were artificially inoculated with 100?104 CFU/25 g of L. monocytogenes and S. Typh-imurium. Citrobacter freundii and Listeria innocua were used as competitive microflora. After enrichment, the sam-ples were analyzed using 3M MDA 2 and real-time PCR. All samples inoculated at concentrations of 100?104 CFU/ 25 g without competitive microflora were positive for S. Typhimurium and L. monocytogenes, as detected by 3M MDA 2 and Korea Standard Food Codex (KFSC) Method. In addition, part of the samples were positive for the pres-ence of C. freundii and L. innocua. The 3M MDA 2 ? Salmonella and Korea Standard Food Codex (KFSC) Method showed similar detection performances in Yuk-hwe and Yuk-sashimi. The 3M MDA 2 method for Salmonella and Listeria, which is a LAMP-based technology, can be used for rapid detection of S. Typhimurium and L. monocyto-genes in raw beef. LAMP bioluminescence assays provide results on the subsequent day and are simple to use com-pared with the Korea Standard Food Codex (KFSC) Method, particularly in terms of DNA preparation.
|
|
|
KEYWORD
|
|
|
3M Molecular Detection Assay 2, Korea Standard Food Codex, Rapid detection, Validation, S. Typhimurium, L. monocytogenes
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|